Energy requirements for specific pair formation during conjugation in Escherichia coli K-12

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Energy requirements for specific pair formation during conjugation in Escherichia coli K-12.

are pre-endowed with appendages, called donor pili, which are responsible for specific pair formation (C. C. Brinton, Trans. N.Y. Acad. Sci. 27:1003, 1965); this led us to believe that specific pair formation might occur in the absence of energy metabolism. However, K. W. Fisher (J. Gen. Microbiol. 16:136, 1957), in showing that zygote formation was dependent on active energy metabolism, conclu...

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Parental functions during conjugation in Escherichia coli K-12.

INTRoDucTioN................................................................ 320 EXPERIMENTAL APPROACH...................................................... 321 MATERIALS AND METHODS..................................................... 321 Media................................................................... 321 Bacterial Strains............................................................ 32...

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Requirements for replication restart proteins during constitutive stable DNA replication in Escherichia coli K-12.

Constitutive stable DNA replication (cSDR) is a mechanism for replisome loading in Escherichia coli K-12. This occurs in a dnaA-independent fashion in an rnhA mutant. cSDR is dependent on recA, priA, and transcription. In this report, it is shown that dnaA rnhA mutants using cSDR for initiation of their DNA replication additionally require priB, but not priC, for viability. Two subtle priA miss...

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Transduction in Escherichia Coli K-12.

SYSTEM of genetic transduction has been discovered in the sexually fertile A K-12 strain of Escherichia coli. This transduction is mediated by lambda, a temperate phage for which K-12 is normally lysogenic. The distinctive features of the lambda-K-12 system include the following: (1) The transductions are limited to a cluster of genes for galactose fermentation. The GaZ loci are closely linked ...

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Modification of conjugation in Escherichia coli K-12 by ultraviolet irradiation.

ONJUGATION in Escherichia coli K-12 results from a sequence of consecutive steps: (1) an effective union between two cells of opposite mating types; (2) a polarized transfer of at least a portion of a male chromosome into the recipient cell, the precise order of transfer depending only on the donor strain utilized; (3) an integration of the genetic information from both parental strains into a ...

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ژورنال

عنوان ژورنال: Journal of Bacteriology

سال: 1967

ISSN: 0021-9193,1098-5530

DOI: 10.1128/jb.94.2.490-492.1967